Filter Paper Dot-Immunobinding Assay for Detection of Spiroplasma citri
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چکیده
منابع مشابه
Latex Agglutination as a Rapid Detection Assay for Spiroplasma citri
Antiserum production. Antiserum was Fletcher, J., and Slack, S. A. 1986. Latex agglutination as a rapid detection assay for Spiroplasma produced in New Zealand white ra 3 citri. Plant Disease 70:754-756. against Illinois horseradish isolates and BR6 as described (5). Antiserum titer The latex agglutination test (LAT) was effective in detecting the wall-less mollicute Spiroplasma was 1: 16,000 b...
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A procedure is described for the routine laboratory diagnosis of viral serum antibodies. Antigens are dotted on nitrocellulose strips or sheets, and sera are applied on absorbent paper strips. Antigen-antibody complexes are detected with enzyme-conjugated antiglobulin and development of a colored, insoluble substrate product. The test allows processing of multiple sera in one 3- to 5-h operatio...
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A rapid and specific dot-enzyme immunoassay (DIBA) using monoclonal antibody was introduced for detection of Trichomonas vaginalis antigen in vaginal and urethral materials. The results of DIBA were compared with culture findings of the parasite in 245 female patients. Taking culture as the reference method, DIBA had a sensitivity of 92% and a specificity of 93%. The predictive value of a posit...
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A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in I-labeled protein A or I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. S...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1987
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.53.1.183-184.1987